Journal: NPJ biofilms and microbiomes

Article Title: Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling.

doi: 10.1038/s41522-023-00415-2

Figure Lengend Snippet: Fig. 2 Liver transcriptome analysis of L. reuteri-treated darkness rats. Heatmaps displaying 36 highly expressed genes (a) and 76 lowly expressed genes (b) in the liver of darkness rats compared with control and DL.reuteri rats through DEG analysis using Ballgown software (| fold change | > 0.6 in the log2 ratio value, raw P < 0.05). c Top terms from GO analysis (above) and terms from KEGG analysis (below) of the 112 differential genes in the DEG analysis. The P values of GO and KEGG analyses were determined on the DAVID website. d Visualization of the top GO and KEGG terms related with lipid metabolism and circadian rhythm in the DEG analysis. e Spearman rank correlations between module eigengenes (ME) and clinical biochemical index in the WGCNA analysis (|ρ | > 0.3, *P < 0.05). f Visualization of the top GO and KEGG terms related with lipid metabolism and circadian rhythm of purple module genes (102) in the WGCNA analysis. Circle, genes; Square, KEGG terms; Hexagon, GO terms. The bigger the square or hexagon, the more genes involved. g Crosstalk among different groups of genes identified the possible target genes taking essential roles in the lipid metabolism of L. reuteri-treated darkness rats. h mRNA abundances of Galr1, Galr2, Nr1d1, Nr1d2, Insig2, Srebf1, Lxra, and Rxra in rat liver detected by qPCR. β-Actin was used as a loading control for qPCR analyses. i Serum galanin concentration detected by ELISA. Statistical analysis (h, i) was performed with one-way ANOVA followed by Newman–Keuls multiple comparison test. n = 8 per group. Data present means ± SEM. *P < 0.05, **P < 0.01.

Article Snippet: Published in partnership with Nanyang Technological University npj Biofilms and Microbiomes (2023) 47 GALR2 antibody (1:500; #26459-1-AP, Proteintech, Wuhan, China), NR1D1 antibody (1:1000, #13418 S, Cell Signaling Technology, Massachusetts, USA; 1:1000, #14506-1-AP, Proteintech), NR1D2 antibody (1:500; #13906-1-AP, Proteintech), SREBP1 antibody (1:1000; #41878, Signalway Antibody), LXRa antibody (1:1000; #ab176323, Abcam, Cambridge, UK), RXRa antibody (1:1000; #ab125001, Abcam), INSIG2 antibody (1:500; #24766-1, Proteintech), P-AKT antibody (1:1000; #4060, Cell Signaling Technology), Fig. 8 Proposed mechanisms for the amelioration of L. reuteri on dyslipidemia in circadian dysrhythmia-induced PCOS-like rats.

Techniques: Control, Software, Concentration Assay, Enzyme-linked Immunosorbent Assay, Comparison

Journal: NPJ biofilms and microbiomes

Article Title: Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling.

doi: 10.1038/s41522-023-00415-2

Figure Lengend Snippet: Fig. 8 Proposed mechanisms for the amelioration of L. reuteri on dyslipidemia in circadian dysrhythmia-induced PCOS-like rats. Left, circadian dysrhythmia due to constant darkness resulted in dyslipidemia and reproductive hallmarks of PCOS in rats. Elevated galanin-GALR1 induced by darkness exposure functioned as an upstream factor of PI3K/AKT pathway and further suppressed NR1D1-induced SREBF1 transcription and translation, thus inducing hepatic lipid accumulation in PCOS-like rats. Right, L. reuteri supplementation ameliorated dyslipidemia and reproductive hallmarks in circadian dysrhythmia-induced PCOS-like rats. L. reuteri restructured microbiome-metabolome network in darkness rats ameliorating the abundance of Lactobacillus, Clostridium sensu stricto 1, Ruminococcaceae UCG-010, and Family XIII AD3011 group, followed by varied serum levels of cortisol, cis-9-palmitoleic acid, 13-methylmyristic acid, capric acid, and dUMP. Notably, capric acid mediated the inhibition of L. reuteri on hepatic GALR1-PI3K/AKT-NR1D1-SREBP1 pathway, which eventually alleviated dyslipidemia.

Article Snippet: Published in partnership with Nanyang Technological University npj Biofilms and Microbiomes (2023) 47 GALR2 antibody (1:500; #26459-1-AP, Proteintech, Wuhan, China), NR1D1 antibody (1:1000, #13418 S, Cell Signaling Technology, Massachusetts, USA; 1:1000, #14506-1-AP, Proteintech), NR1D2 antibody (1:500; #13906-1-AP, Proteintech), SREBP1 antibody (1:1000; #41878, Signalway Antibody), LXRa antibody (1:1000; #ab176323, Abcam, Cambridge, UK), RXRa antibody (1:1000; #ab125001, Abcam), INSIG2 antibody (1:500; #24766-1, Proteintech), P-AKT antibody (1:1000; #4060, Cell Signaling Technology), Fig. 8 Proposed mechanisms for the amelioration of L. reuteri on dyslipidemia in circadian dysrhythmia-induced PCOS-like rats.

Techniques: Inhibition

Journal: Journal of radiation research

Article Title: Combining network pharmacology and in vitro and in vivo experiments to study the mechanism of Keluoxin in the treatment of radiation nephropathy†.

doi: 10.1093/jrr/rrad050

Figure Lengend Snippet: Fig. 1. The network of Keluoxin in the treatment of radiation nephropathy. (A) Venn diagram of the intersecting targets of Keluoxin and radiation nephropathy. (B) The compound-target network of Keluoxin. (C) The interaction network of 50 key target proteins. (D) The compound-target-pathway network.

Article Snippet: Foetal bovine serum, phosphate buffer solution (PBS), DMEM, a penicillin and streptomycin mixture, Cell Counting Kit 8 (CCK-8) kits and lactate dehydrogenase (LDH) kits were purchased from Beijing Solabo Technology Co, Ltd. creatinine (CR), blood urea nitrogen (BUN), IL-6, TNF-α, TGF-β and IFN-γ kits were purchased from Shanghai Biyuntian Technology Co, Ltd. Trypsin, JAK1, JAK2, STAT1, STAT3 and HIF-1α antibodies were purchased from Proteintech (Wuhan, China).

Techniques:

Journal: Journal of radiation research

Article Title: Combining network pharmacology and in vitro and in vivo experiments to study the mechanism of Keluoxin in the treatment of radiation nephropathy†.

doi: 10.1093/jrr/rrad050

Figure Lengend Snippet: Fig. 4. Effect of Keluoxin on lipid peroxidation of TCMK-1 after X-ray irradiation. (A, B) Effect of serum-containing Keluoxin on MDA and GSH levels after X-ray irradiation. After 10 Gy irradiation, the expression of MDA increased and the expression of GSH decreased in TCMK-1 cells, with statistical differences (all compared with CON groups). And medicated serum-containing Keluoxin can inhibit these changes.

Article Snippet: Foetal bovine serum, phosphate buffer solution (PBS), DMEM, a penicillin and streptomycin mixture, Cell Counting Kit 8 (CCK-8) kits and lactate dehydrogenase (LDH) kits were purchased from Beijing Solabo Technology Co, Ltd. creatinine (CR), blood urea nitrogen (BUN), IL-6, TNF-α, TGF-β and IFN-γ kits were purchased from Shanghai Biyuntian Technology Co, Ltd. Trypsin, JAK1, JAK2, STAT1, STAT3 and HIF-1α antibodies were purchased from Proteintech (Wuhan, China).

Techniques: Irradiation, Expressing

Journal: Journal of radiation research

Article Title: Combining network pharmacology and in vitro and in vivo experiments to study the mechanism of Keluoxin in the treatment of radiation nephropathy†.

doi: 10.1093/jrr/rrad050

Figure Lengend Snippet: Fig. 3. Effect of different doses of X-ray on the activity of TCMK-1 cells. (A) TCMK-1 cell mortality after different doses of X-ray irradiation. Measure cell mortality 48 h after X-ray irradiation. As the X-ray dose increases, the cell mortality rate gradually increases. (B) TCMK-1 cell viability at 12, 24 and 48 h after different doses of X-ray irradiation. Measure cell mortality 12, 24 and 48 h after X-ray irradiation. As the X-ray dose increases, the cell survival rate gradually decreases. The most significant decrease in cell viability was observed 48 h after 10 Gy X-ray irradiation. (C) Effect of medicated serum containing Keluoxin on TCMK-1 cell viability. As the X-ray dose increases, the cell survival rate gradually decreases and medicated serum-containing Keluoxin can inhibit this decline (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).

Article Snippet: Foetal bovine serum, phosphate buffer solution (PBS), DMEM, a penicillin and streptomycin mixture, Cell Counting Kit 8 (CCK-8) kits and lactate dehydrogenase (LDH) kits were purchased from Beijing Solabo Technology Co, Ltd. creatinine (CR), blood urea nitrogen (BUN), IL-6, TNF-α, TGF-β and IFN-γ kits were purchased from Shanghai Biyuntian Technology Co, Ltd. Trypsin, JAK1, JAK2, STAT1, STAT3 and HIF-1α antibodies were purchased from Proteintech (Wuhan, China).

Techniques: Activity Assay, Irradiation